Interdigitated ITO sensor for ECIS monitoring of breast cancer cells

Abstract : In the fight against cancer, personalized medicine is a necessary and very promising strategy. In fact, it is essential to be able to test the safety and effectiveness of anticancer drugs on samples from the patient, due to the diversity of responses between patients. The aim is to improve the performance of health care and avoid unnecessary and sometimes harmful treatments. Thus, chemotherapy perfectly illustrates this strategy. The high cost of therapeutic molecules, the harmfulness of these molecules and the varied responses of patients involve the use of tests with chemotherapeutic molecules on samples coming from cancer patients. This results in a growing interest in the development of simple, robust and inexpensive tests for assessing the chemo sensitivity of biological cells from a biopsy. The problems related to carrying out such tests are the quantity of cells available in a biopsy, the diversity of the therapeutic molecules to be tested and also the choice of a detection technique, able to monitor the kinetics of action of the molecules on the biological cells. One solution to the small amount of cells is to carry out the tests in microfluidic environments which therefore require the integration and miniaturization of a detection technique. The strategy that will be studied in this thesis is the use of electrical impedance with interdigitated electrodes of indium tin oxide (ITO) for the quantitative analysis of the state of breast cancer cells for screening applications of anticancer drugs. This material has the advantage of being transparent allowing impedance measurements that could be coupled to optical measurements in a microfluidic environment. In the first part, we characterized and studied interdigitated electrodes of gold and ITO for impedance measurements with cancer cells. This impedance spectroscopy characterization carried out in culture medium solutions, in the presence and absence of cells, demonstrated that the difference in sensitivity between these two materials comes from a difference in resistive behavior and also from a difference in interfacial impedance, in both cases to the disadvantage of ITO. After this, we continued the study to evaluate the capabilities of ITO for chemosensitivity measurements using the molecule 5 fluorouracil and we suggested a strategy to improve the sensitivity of ITO while maintaining its transparency. The strategy developed consists of modifying the surface of ITO electrodes with iridium oxide to improve the sensitivity of the ITO, while keeping its transparency. This approach is interesting for developing a transparent device and easy to couple with a microscopic observation system in a microfluidic environment
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Jaime Andres Martinez Santamaria. Interdigitated ITO sensor for ECIS monitoring of breast cancer cells. Biotechnology. Université de Lyon, 2019. English. ⟨NNT : 2019LYSE1005⟩. ⟨tel-02125936⟩

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