Skip to Main content Skip to Navigation
Journal articles

High Performance Thin-Layer Chromatography of Plant Ecdysteroids Coupled with Desorption Electrospray Ionisation-Ion Mobility-Time of Flight High Resolution Mass Spectrometry (HPTLC/DESI/IM/ToFMS)

Abstract : The use of high performance thin-layer chromatography (HPTLC) in combination with high resolution time of flight mass spectrometry (MS) for the detection, identification and imaging (HPTLC/MSI) of ecdysteroids (insect moulting hormones) present in a number of plant extracts obtained from members of the Silene family is demonstrated. DESI is shown to be a convenient method for the recovery of these polar polyhydroxylated steroids from the silica gel of the HPTLC plate for subsequent MS detection and imaging. The incorporation of an ion mobility separation (IMS) to the system to give HPTLC/IMS/ MS provided additional drift time data which enabled more confident identification. Using HPTLC/DESI/IMS/MS, a range of ecdysteroids were detected and characterized in extracts of S. otitis, S nutans, S. maritime, S. viridiflora and S. fimbriata.
Document type :
Journal articles
Complete list of metadata

Cited literature [15 references]  Display  Hide  Download

https://hal.sorbonne-universite.fr/hal-02878364
Contributor : Hal Sorbonne Université Gestionnaire Connect in order to contact the contributor
Submitted on : Tuesday, June 23, 2020 - 10:00:50 AM
Last modification on : Saturday, December 4, 2021 - 3:58:36 AM
Long-term archiving on: : Thursday, September 24, 2020 - 3:33:19 PM

File

Claude2020_Article_HighPerform...
Publication funded by an institution

Identifiers

Citation

E. Claude, M. Tower, R. Lafont, I.D. Wilson, R.S. Plumb. High Performance Thin-Layer Chromatography of Plant Ecdysteroids Coupled with Desorption Electrospray Ionisation-Ion Mobility-Time of Flight High Resolution Mass Spectrometry (HPTLC/DESI/IM/ToFMS). Chromatographia, Springer Verlag, 2020, ⟨10.1007/s10337-020-03917-9⟩. ⟨hal-02878364⟩

Share

Metrics

Record views

82

Files downloads

178