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Boosting functionality of synthetic DNA circuits with tailored deactivation

Abstract : Molecular programming takes advantage of synthetic nucleic acid biochemistry to assemble networks of reactions, in vitro, with the double goal of better understanding cellular regulation and providing information-processing capabilities to man-made chemical systems. The function of molecular circuits is deeply related to their topological structure, but dynamical features (rate laws) also play a critical role. Here we introduce a mechanism to tune the nonlinearities associated with individual nodes of a synthetic network. This mechanism is based on programming deactivation laws using dedicated saturable pathways. We demonstrate this approach through the conversion of a single-node homoeostatic network into a bistable and reversible switch. Furthermore, we prove its generality by adding new functions to the library of reported man-made molecular devices: a system with three addressable bits of memory, and the first DNA-encoded excitable circuit. Specific saturable deactivation pathways thus greatly enrich the functional capability of a given circuit topology.
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Submitted on : Monday, December 19, 2016 - 12:27:47 PM
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Kevin Montagne, Guillaume Gines, Teruo Fujii, Yannick Rondelez. Boosting functionality of synthetic DNA circuits with tailored deactivation. Nature Communications, Nature Publishing Group, 2016, 7, ⟨10.1038/ncomms13474⟩. ⟨hal-01419361⟩



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