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A subtelomeric region affects telomerase-negative replicative senescence in Saccharomyces cerevisiae

Abstract : In eukaryotes, telomeres determine cell proliferation potential by triggering replicative senescence in the absence of telomerase. In Saccharomyces cerevisiae, senescence is mainly dictated by the first telomere that reaches a critically short length, activating a DNA-damage-like response. How the corresponding signaling is modulated by the telomeric structure and context is largely unknown. Here we investigated how subtelomeric elements of the shortest telomere in a telomerase-negative cell influence the onset of senescence. We found that a 15 kb truncation of the 7L subtelomere widely used in studies of telomere biology affects cell growth when combined with telomerase inactivation. This effect is likely not explained by (i) elimination of sequence homology at chromosome ends that would compromise homology-directed DNA repair mechanisms; (ii) elimination of the conserved subtelomeric X-element; (iii) elimination of a gene that would become essential in the absence of telomerase; and (iv) heterochromatinization of inner genes, causing the silencing of an essential gene in replicative senescent cells. This works contributes to better delineate subtelomere functions and their impact on telomere biology.
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https://hal.archives-ouvertes.fr/hal-02325822
Contributor : Zhou Xu Connect in order to contact the contributor
Submitted on : Friday, December 4, 2020 - 10:32:56 AM
Last modification on : Thursday, May 19, 2022 - 1:52:03 PM

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Pascale Jolivet, Kamar Serhal, Marco Graf, Stephan Eberhard, Zhou Xu, et al.. A subtelomeric region affects telomerase-negative replicative senescence in Saccharomyces cerevisiae. Scientific Reports, Nature Publishing Group, 2019, 9 (1), ⟨10.1038/s41598-018-38000-9⟩. ⟨hal-02325822⟩

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