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Contribution à l'amélioration de la conservation cornéenne en bioréacteur

Abstract : Since the abandonment for logistical and health security reasons in the 70s-80 of fresh grafts whose longevity exceeded two decades, the technical storage was organized around two techniques of eye banking: hypothermia (short term storage) in the USA and organ culture (long term storage) in Europe. Besançon was a pioneer in France for this technique. Induced stromal edema associated with these passive techniques leads to a significant endothelial cell loss and reduces grafts survival, limits quality control and obstructs further developments such as pre-cut endothelial graft or endothelial bioengineering.The laboratory "Biology, Imaging and Engineering of the Corneal Graft (BiiGC, EA 2521, Jean Monnet University of St-Etienne) has developed an active storage system, the bioreactor, that limits corneal edema by restoring a pressure corneal gradient and circulation of the storage medium. Bioreactor makes possible to overcome these two major disadvantages and to implement the developments of the future.The aim of this thesis is to 1 / trace the evolution of storage and control techniques of corneal graft; 2 / review the evolution of corneal graft techniques / indications in France; 3 / document early endothelial cell loss related to passive conservation in organ culture; 4 / Compare organ culture versus bioreactor storage.Key words: corneal storage, eye bank, endothelial viability, organ culture, bioreactor, graft survival
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Submitted on : Tuesday, May 11, 2021 - 3:56:09 PM
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Anne-Sophie Gauthier. Contribution à l'amélioration de la conservation cornéenne en bioréacteur. Médecine humaine et pathologie. Université Bourgogne Franche-Comté, 2018. Français. ⟨NNT : 2018UBFCE011⟩. ⟨tel-03224330⟩

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