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Journal articles
Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy
Irene Pila-Castellanos
1, 2
Diana Molino
2
Joe Mckellar
3
Laetitia Lines
1
Juliane da Graca
2
Marine Tauziet
3
Laurent Chanteloup
1
Ivan Mikaelian
4, 5
Laurène Meyniel-Schicklin
1
Patrice Codogno
2
Jacky Vonderscher
1
Cédric Delevoye
6
Olivier Moncorgé
4, 5
Eric Meldrum
1
Caroline Goujon
4, 5
Etienne Morel
2
Benoit de Chassey
1
Irene Pila-Castellanos 1, 2
Author ORCID : https://orcid.org/0000-0002-7400-5088
Diana Molino 2
Author
Joe Mckellar 3
Author ORCID : https://orcid.org/0000-0002-5440-2879
Laetitia Lines 1
Author
Juliane da Graca 2
Author ORCID : https://orcid.org/0000-0002-1879-617X
Marine Tauziet 3
Author ORCID : https://orcid.org/0000-0001-7924-0055
Laurent Chanteloup 1
Author ORCID : https://orcid.org/0000-0001-9715-7204
Ivan Mikaelian 4, 5
Author Employer institution : Centre National de la Recherche Scientifique
Laurène Meyniel-Schicklin 1
Author
Patrice Codogno 2
Author ORCID : https://orcid.org/0000-0002-5492-3180
Jacky Vonderscher 1
Author ORCID : https://orcid.org/0000-0003-4963-5632
Cédric Delevoye 6
Author ORCID : https://orcid.org/0000-0002-3835-6649
Olivier Moncorgé 4, 5
Author ORCID : https://orcid.org/0000-0003-0512-1824
Eric Meldrum 1
Author
Caroline Goujon 4, 5
Author ORCID : https://orcid.org/0000-0001-8571-1108
Etienne Morel 2
Author
Benoit de Chassey 1
Author Employer institution : ENYO Pharma SAS ORCID : https://orcid.org/0000-0002-7488-8597
1
ENYO Pharma SAS (Lyon - France)
2
INEM - UM 111 (UMR 8253 / U1151) - Institut Necker Enfants-Malades (Bâtiment Leriche
14 rue Maria Helena Vieira Da Silva
75014 Paris - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1151 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 8253 (France)
- UP - Université de Paris : UM 111 (85 boulevard Saint-Germain 75006 Paris - France)
3
IRIM - Institut de Recherche en Infectiologie de Montpellier (UMR 9004, 1919 Route de Mende - 34293 Montpellier - France)
- UM - Université de Montpellier (163 rue Auguste Broussonnet - 34090 Montpellier - France)
- CNRS - Centre National de la Recherche Scientifique : UMR9004 (France)
4
UNICANCER/CRCL - Centre de Recherche en Cancérologie de Lyon (28, rue Laennec - 69373 Lyon - France)
- Centre Léon Bérard [Lyon] (28, rue Laennec 69373 LYON Cedex 08 - France)
- UCBL - Université Claude Bernard Lyon 1 (43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex - France)
- Université de Lyon (92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5286 (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1052 (101, rue de Tolbiac, 75013 Paris - France)
5
Centre Léon Bérard [Lyon] (28, rue Laennec 69373 LYON Cedex 08 - France)
6
Biologie Cellulaire et Cancer (26 Rue d'Ulm 75248 PARIS CEDEX 05 - France)
- Institut Curie [Paris] ( 26 rue d'Ulm - 75248 Paris - France)
- SU - Sorbonne Université (21 rue de l’École de médecine - 75006 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR144 (France)
Abstract : Influenza virus infections are major public health threats due to their high rates of morbidity and mortality. Upon influenza virus entry, host cells experience modifications of endomembranes, including those used for virus trafficking and replication. Here we report that influenza virus infection modifies mitochondrial morphodynamics by promoting mitochondria elongation and altering endoplasmic reticulum-mitochondria tethering in host cells. Expression of the viral RNA recapitulates these modifications inside cells. Virus induced mitochondria hyper-elongation was promoted by fission associated protein DRP1 relocalization to the cytosol, enhancing a profusion status. We show that altering mitochondrial hyper-fusion with Mito-C, a novel pro-fission compound, not only restores mitochondrial morphodynamics and endoplasmic reticulum-mitochondria contact sites but also dramatically reduces influenza replication. Finally, we demonstrate that the observed Mito-C antiviral property is directly connected with the innate immunity signaling RIG-I complex at mitochondria. Our data highlight the importance of a functional interchange between mitochondrial morphodynamics and innate immunity machineries in the context of influenza viral infection.
Document type :
Journal articles
Complete list of metadata
https://hal.archives-ouvertes.fr/hal-03439550
Contributor : Ivan Mikaelian Connect in order to contact the contributor
Submitted on : Monday, November 22, 2021 - 12:00:09 PM
Last modification on : Wednesday, January 12, 2022 - 12:18:02 PM
Contributor : Ivan Mikaelian Connect in order to contact the contributor
Submitted on : Monday, November 22, 2021 - 12:00:09 PM
Last modification on : Wednesday, January 12, 2022 - 12:18:02 PM
File
journal.ppat.1009340 (1).pdf
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Identifiers
- HAL Id : hal-03439550, version 1
- DOI : 10.1371/journal.ppat.1009340
- PUBMED : 33596274
- PUBMEDCENTRAL : PMC7920353
Collections
PSL | BS | UNIV-LYON1 | UNIV-MONTPELLIER | CRCL | UDL | UP-SANTE | CNRS | UNIV-PARIS | CURIE | FNCLCC | UMR144 | SU-SCIENCES | SORBONNE-UNIVERSITE | SU-TI
Citation
Irene Pila-Castellanos, Diana Molino, Joe Mckellar, Laetitia Lines, Juliane da Graca, et al.. Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy. PLoS Pathogens, Public Library of Science, 2021, 17, ⟨10.1371/journal.ppat.1009340⟩. ⟨hal-03439550⟩
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