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Live-cell micromanipulation of a genomic locus reveals interphase chromatin mechanics

Abstract : Our understanding of the physical principles organizing the genome in the nucleus is limited by the lack of tools to directly exert and measure forces on interphase chromosomes in vivo and probe their material nature. Here, we present a novel approach to actively manipulate a genomic locus using controlled magnetic forces inside the nucleus of a living human cell. We observe viscoelastic displacements over microns within minutes in response to near-picoNewton forces, which are well captured by a Rouse polymer model. Our results highlight the fluidity of chromatin, with a moderate contribution of the surrounding material, revealing the minor role of crosslinks and topological effects and challenging the view that interphase chromatin is a gel-like material. Our new technology opens avenues for future research, from chromosome mechanics to genome functions.
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https://hal.archives-ouvertes.fr/hal-03437297
Contributor : Antoine Coulon Connect in order to contact the contributor
Submitted on : Friday, November 19, 2021 - 6:16:57 PM
Last modification on : Sunday, June 26, 2022 - 3:20:34 AM
Long-term archiving on: : Sunday, February 20, 2022 - 9:06:54 PM

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Veer Keizer, Simon Grosse-Holz, Maxime Woringer, Laura Zambon, Koceila Aizel, et al.. Live-cell micromanipulation of a genomic locus reveals interphase chromatin mechanics. 2021. ⟨hal-03437297⟩

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