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Microvilli-derived Extracellular Vesicles Govern Morphogenesis in Drosophila wing epithelium

Abstract : The regulation and coordination of developmental processes involves the secretion of morphogens and membrane carriers, including extracellular vesicles, which facilitate their transport over long distance. The long-range activity of the Hedgehog morphogen is conveyed by extracellular vesicles. However, the site and the molecular basis of their biogenesis remains unknown. By combining fluorescence and electron microscopy combined with genetics and cell biology approaches, we investigated the origin and the cellular mechanisms underlying extracellular vesicle biogenesis, and their contribution to Drosophila wing disc development, exploiting Hedgehog as a long-range morphogen. We show that microvilli of Drosophila wing disc epithelium are the site of generation of small extracellular vesicles that transport Hedgehog across the tissue. This process requires the Prominin-like protein, whose activity, together with interacting cytoskeleton components and lipids, is critical for maintaining microvilli integrity and function in secretion. Our results provide the first evidence that microvilli-derived extracellular vesicles contribute to Hedgehog long-range signaling activity highlighting their physiological significance in tissue development in vivo .
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Preprints, Working Papers, ...
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https://hal.archives-ouvertes.fr/hal-03414813
Contributor : Gisela d'Angelo Connect in order to contact the contributor
Submitted on : Thursday, November 4, 2021 - 4:27:51 PM
Last modification on : Wednesday, January 12, 2022 - 12:18:02 PM

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2020.11.01.363697.full.pdf
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Ilse Hurbain, Anne-Sophie Macé, Maryse Romao, Lucie Sengmanivong, Laurent Ruel, et al.. Microvilli-derived Extracellular Vesicles Govern Morphogenesis in Drosophila wing epithelium. 2021. ⟨hal-03414813⟩

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