Skip to Main content Skip to Navigation
Journal articles

The GTPase Rab8 differentially controls the long- and short-range activity of the Hedgehog morphogen gradient by regulating Hedgehog apico-basal distribution

Abstract : ABSTRACT The Hedgehog (Hh) morphogen gradient is required for patterning during metazoan development, yet the mechanisms involved in Hh apical and basolateral release and how this influences short- and long-range target induction are poorly understood. We found that depletion of the GTPase Rab8 in Hh-producing cells induces an imbalance between the level of apically and laterally released Hh. This leads to non-cell-autonomous differential effects on the expression of Hh target genes, namely an increase in its short-range targets and a concomitant decrease in long-range targets. We further found that Rab8 regulates the endocytosis and apico-basal distribution of Ihog, a transmembrane protein known to bind to Hh and to be crucial for establishment of the Hh gradient. Our data provide new insights into morphogen gradient formation, whereby morphogen activity is functionally distributed between apically and basolaterally secreted pools.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-03414597
Contributor : Gisela D'ANGELO Connect in order to contact the contributor
Submitted on : Monday, November 8, 2021 - 1:26:17 PM
Last modification on : Thursday, August 4, 2022 - 4:56:19 PM
Long-term archiving on: : Wednesday, February 9, 2022 - 7:45:10 PM

File

dev191791.pdf
Explicit agreement for this submission

Identifiers

Citation

Tanvi Gore, Tamás Matusek, Gisela d'Angelo, Cécile Giordano, Thomas Tognacci, et al.. The GTPase Rab8 differentially controls the long- and short-range activity of the Hedgehog morphogen gradient by regulating Hedgehog apico-basal distribution. Development (Cambridge, England), Company of Biologists, 2021, 148 (5), ⟨10.1242/dev.191791⟩. ⟨hal-03414597⟩

Share

Metrics

Record views

49

Files downloads

2