Skip to Main content Skip to Navigation

Cyclin B3 promotes anaphase I onset in oocyte meiosis

Abstract : Meiosis poses unique challenges because two rounds of chromosome segregation must be executed without intervening DNA replication. Mammalian cells express numerous temporally regulated cyclins, but how these proteins collaborate to control meiosis remains poorly understood. Here, we show that female mice genetically ablated for cyclin B3 are viable-indicating that the protein is dispensable for mitotic divisions-but are sterile. Mutant oocytes appear normal until metaphase I but then display a highly penetrant failure to transition to anaphase I. They arrest with hallmarks of defective anaphase-promoting complex/cyclosome (APC/C) activation, including no separase activity, high CDK1 activity, and high cyclin B1 and securin levels. Partial APC/C activation occurs, however, as exogenously expressed APC/C substrates can be degraded. Cyclin B3 forms active kinase complexes with CDK1, and meiotic progression requires cyclin B3-associated kinase activity. Cyclin B3 homologues from frog, zebrafish, and fruit fly rescue meiotic progression in cyclin B3-deficient mouse oocytes, indicating conservation of the biochemical properties and possibly cellular functions of this germline-critical cyclin.
Complete list of metadatas

Cited literature [58 references]  Display  Hide  Download

https://hal.sorbonne-universite.fr/hal-02171206
Contributor : Gestionnaire Hal-Su <>
Submitted on : Tuesday, July 2, 2019 - 5:04:55 PM
Last modification on : Tuesday, July 9, 2019 - 4:55:31 PM

File

Karasu, Bouftas et al small.pd...
Files produced by the author(s)

Identifiers

Citation

Mehmet Karasu, Nora Bouftas, Katja Wassmann, Scott Keeney. Cyclin B3 promotes anaphase I onset in oocyte meiosis. Journal of Cell Biology, Rockefeller University Press, 2019, 218 (4), pp.1265-1281. ⟨10.1083/jcb.201808091⟩. ⟨hal-02171206⟩

Share

Metrics

Record views

71

Files downloads

104