Skip to Main content Skip to Navigation
Preprints, Working Papers, ...

pH fluctuations drive waves of stereotypical cellular reorganizations during entry into quiescence

Abstract : The life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive establishment of structural reorganizations observed in quiescent cells remains unclear. To address this question, we have developed a method that combines nutrient-limited proliferation assays at the population level with single-cell tracking to unravel the coordination between metabolic and structural transitions in cells during an unperturbed lifecycle. We show that non-monotonous internal pH fluctuations are in sync with successive waves of protein super-assemblies formation and ultimately lead to a cytosolic glass transition. Our results, therefore, suggest a simple model explaining how the complex developmental changes during the yeast life cycle are orchestrated by the sequence of metabolic transitions.
Document type :
Preprints, Working Papers, ...
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-03096906
Contributor : Charvin Gilles <>
Submitted on : Thursday, January 7, 2021 - 12:26:54 PM
Last modification on : Friday, January 8, 2021 - 8:38:16 AM

File

Q05 (1).pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Basile Jacquel, Théo Aspert, Damien Laporte, Isabelle Sagot, Gilles Charvin. pH fluctuations drive waves of stereotypical cellular reorganizations during entry into quiescence. 2021. ⟨hal-03096906⟩

Share

Metrics

Record views

14

Files downloads

46