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Proteostasis collapse, a hallmark of aging, hinders the chaperone-Start network and arrests cells in G1

Abstract : Loss of proteostasis and cellular senescence are key hallmarks of aging, but direct cause-effect relationships are not well understood. We show that most yeast cells arrest in G1 before death with low nuclear levels of Cln3, a key G1 cyclin extremely sensitive to chaperone status. Chaperone availability is seriously compromised in aged cells, and the G1 arrest coincides with massive aggregation of a metastable chaperone-activity reporter. Moreover, G1-cyclin overexpression increases lifespan in a chaperone-dependent manner. As a key prediction of a model integrating autocatalytic protein aggregation and a minimal Start network, enforced protein aggregation causes a severe reduction in lifespan, an effect that is greatly alleviated by increased expression of specific chaperones or cyclin Cln3. Overall, our data show that proteostasis breakdown, by compromising chaperone activity and G1-cyclin function, causes an irreversible arrest in G1, configuring a molecular pathway postulating proteostasis decay as a key contributing effector of cell senescence.
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https://hal.archives-ouvertes.fr/hal-03096864
Contributor : Charvin Gilles <>
Submitted on : Thursday, January 7, 2021 - 11:45:14 AM
Last modification on : Thursday, January 7, 2021 - 11:47:30 AM

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David Moreno, Kirsten Jenkins, Sandrine Morlot, Gilles Charvin, Attila Csikasz-Nagy, et al.. Proteostasis collapse, a hallmark of aging, hinders the chaperone-Start network and arrests cells in G1. eLife, eLife Sciences Publication, 2019, 8, ⟨10.7554/eLife.48240⟩. ⟨hal-03096864⟩

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