Skip to Main content Skip to Navigation
Journal articles

Stage-specific expression of an odorant receptor underlies olfactory behavioral plasticity in Spodoptera littoralis larvae

Abstract : Background: The detection of environmental cues and signals via the sensory system directs behavioral choices in diverse organisms. Insect larvae rely on input from the chemosensory system, mainly olfaction, for locating food sources. In several lepidopteran species, foraging behavior and food preferences change across larval instars; however, the molecular mechanisms underlying such behavioral plasticity during larval development are not fully understood. Here, we hypothesize that expression patterns of odorant receptors (ORs) change during development, as a possible mechanism influencing instar-specific olfactory-guided behavior and food preferences. Results: We investigated the expression patterns of ORs in larvae of the cotton leafworm Spodoptera littoralis between the first and fourth instar and revealed that some of the ORs show instar-specific expression. We functionally characterized one OR expressed in the first instar, SlitOR40, as responding to the plant volatile, βcaryophyllene and its isomer α-humulene. In agreement with the proposed hypothesis, we showed that first but not fourth instar larvae responded behaviorally to β-caryophyllene and α-humulene. Moreover, knocking out this odorant receptor via CRISPR-Cas9, we confirmed that instar-specific responses towards its cognate ligands rely on the expression of SlitOR40. Conclusion: Our results provide evidence that larvae of S. littoralis change their peripheral olfactory system during development. Furthermore, our data demonstrate an unprecedented instar-specific behavioral plasticity mediated by an OR, and knocking out this OR disrupts larval behavioral plasticity. The ecological relevance of such behavioral plasticity for S. littoralis remains to be elucidated, but our results demonstrate an olfactory mechanism underlying this plasticity in foraging behavior during larval development.
Document type :
Journal articles
Complete list of metadata

https://hal.sorbonne-universite.fr/hal-03420548
Contributor : HAL Sorbonne Université 5 Gestionnaire Connect in order to contact the contributor
Submitted on : Tuesday, November 9, 2021 - 11:24:13 AM
Last modification on : Friday, April 15, 2022 - 3:35:26 AM

File

s12915-021-01159-1.pdf
Publication funded by an institution

Identifiers

Citation

Santosh V Revadi, Vito Antonio Giannuzzi, Valeria Rossi, Gert Martin Hunger, Lucie Conchou, et al.. Stage-specific expression of an odorant receptor underlies olfactory behavioral plasticity in Spodoptera littoralis larvae. BMC Biology, BioMed Central, 2021, 19 (1), ⟨10.1186/s12915-021-01159-1⟩. ⟨hal-03420548⟩

Share

Metrics

Record views

30

Files downloads

27