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Journal articles
Chemo‐Enzymatic Synthesis of S. mansoni O‐Glycans and Their Evaluation as Ligands for C‐Type Lectin Receptors MGL, DC‐SIGN, and DC‐SIGNR
Abstract : Due to their interactions with C-type lectin receptors (CLRs), glycans from the helminth Schistosoma mansoni represent promising leads for treatment of autoimmune diseases, allergies or cancer. We chemo-enzymatically synthesized nine O-glycans based on the two predominant O-glycan cores observed in the infectious stages of schistosomiasis, the mucin core 2 and the S. mansoni core. The O-glycans were fucosylated next to a selection of N-glycans directly on a microarray slide using a recombinant fucosyltransferase and GDP-fucose or GDP-6-azidofucose as donor. Binding assays with fluorescently labelled human CLRs DC-SIGN, DC-SIGNR and MGL revealed the novel O-glycan O8 as the best ligand for MGL from our panel. Significant binding to DC-SIGN was also found for azido-fucosylated glycans. Contrasting binding specificities were observed between the monovalent carbohydrate recognition domain (CRD) and the tetravalent extracellular domain (ECD) of DC-SIGNR.
https://hal.archives-ouvertes.fr/hal-02974726
Contributor : Frank Thomas <>
Submitted on : Wednesday, November 25, 2020 - 11:17:09 AM
Last modification on : Wednesday, November 25, 2020 - 3:59:04 PM
Contributor : Frank Thomas <>
Submitted on : Wednesday, November 25, 2020 - 11:17:09 AM
Last modification on : Wednesday, November 25, 2020 - 3:59:04 PM
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Pham et al. Chem A Eur J.pdf
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