DC-SIGN and L-SIGN are high affinity binding receptors for hepatitis C virus glycoprotein E2. - Groupe Structure et Activité des Glycosaminoglycanes / Structure and Activity of Glycosaminoglycans Group (IBS-SAGAG) Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2003

DC-SIGN and L-SIGN are high affinity binding receptors for hepatitis C virus glycoprotein E2.

Pierre-Yves Lozach
Isabelle Staropoli
Ali Amara
  • Fonction : Auteur
Franck Fieschi
Olivier Schwartz
  • Fonction : Auteur
  • PersonId : 999827
Fernando Arenzana-Seisdedos
  • Fonction : Auteur
  • PersonId : 853031
Ralf Altmeyer
  • Fonction : Auteur

Résumé

The hepatitis C virus (HCV) genome codes for highly mannosylated envelope proteins, which are naturally retained in the endoplasmic reticulum. We found that the HCV envelope glycoprotein E2 binds the dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and the related liver endothelial cell lectin L-SIGN through high-mannose N-glycans. Competing ligands such as mannan and an antibody directed against the carbohydrate recognition domains (CRD) abrogated binding. While no E2 interaction with distant monomeric CRDs on biosensor chips could be detected, binding is observed if CRDs are closely seeded (Kd = 48 nm) and if the CRD is part of the oligomeric-soluble extracellular domain of DC-SIGN (Kd = 30 nm). The highest affinity is seen for plasma membrane-expressed DC-SIGN and L-SIGN (Kd = 3 and 6 nm, respectively). These results indicate that several high-mannose N-glycans in a structurally defined cluster on E2 bind to several subunits of the oligomeric lectin CRD. High affinity interaction of viral glycoproteins with oligomeric lectins might represent a strategy by which HCV targets to and concentrates in the liver and infects dendritic cells.
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Dates et versions

hal-01061433 , version 1 (04-10-2016)

Identifiants

Citer

Pierre-Yves Lozach, Hugues Lortat-Jacob, Agnès de Lacroix de Lavalette, Isabelle Staropoli, Steven Foung, et al.. DC-SIGN and L-SIGN are high affinity binding receptors for hepatitis C virus glycoprotein E2.. Journal of Biological Chemistry, 2003, 278 (22), pp.20358-66. ⟨10.1074/jbc.M301284200⟩. ⟨hal-01061433⟩
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