Skip to Main content Skip to Navigation
Journal articles

Capture at the single cell level of metabolic modules distinguishing aggressive and indolent glioblastoma cells

Abstract : Glioblastoma cell ability to adapt their functioning to microenvironment changes is a source of the extensive intra-tumor heterogeneity characteristic of this devastating malignant brain tumor. A systemic view of the metabolic pathways underlying glioblastoma cell functioning states is lacking. We analyzed public single cell RNA-sequencing data from glioblastoma surgical resections, which offer the closest available view of tumor cell heterogeneity as encountered at the time of patients' diagnosis. Unsupervised analyses revealed that information dispersed throughout the cell transcript repertoires encoded the identity of each tumor and masked information related to cell functioning states. Data reduction based on an experimentally-defined signature of transcription factors overcame this hurdle. It allowed cell grouping according to their tumorigenic potential, regardless of their tumor of origin. The approach relevance was validated using independent datasets of glioblastoma cell and tissue transcriptomes, patient-derived cell lines and orthotopic xenografts. Overexpression of genes coding for amino acid and lipid metabolism enzymes involved in anti-oxidative, energetic and cell membrane processes characterized cells with high tumorigenic potential. Modeling of their expression network highlighted the very long chain polyunsaturated fatty acid synthesis pathway at the core of the network. Expression of its most downstream enzymatic component, ELOVL2, was associated with worsened patient survival, and required for cell tumorigenic properties in vivo. Our results demonstrate the power of signature-driven analyses of single cell transcriptomes to obtain an integrated view of metabolic pathways at play within the heterogeneous cell landscape of patient tumors.
Document type :
Journal articles
Complete list of metadata

Cited literature [76 references]  Display  Hide  Download
Contributor : Gestionnaire HAL-SU Connect in order to contact the contributor
Submitted on : Monday, November 4, 2019 - 1:08:55 PM
Last modification on : Saturday, December 4, 2021 - 4:00:09 AM
Long-term archiving on: : Wednesday, February 5, 2020 - 7:54:19 PM


Publication funded by an institution



Mirca S Saurty-Seerunghen, Léa Bellenger, Elias El-Habr, Virgile Delaunay, Delphine Garnier, et al.. Capture at the single cell level of metabolic modules distinguishing aggressive and indolent glioblastoma cells. Acta Neuropathologica Communications, BioMed Central part of Springer Science, 2019, 7 (1), ⟨10.1186/s40478-019-0819-y⟩. ⟨hal-02344991⟩



Record views


Files downloads